Journal: Scientific Reports

Article Title: An in vitro method to keep human aortic tissue sections functionally and structurally intact

doi: 10.1038/s41598-018-26549-4

Figure Lengend Snippet: Immunofluorescence images using Zeiss Axiovert 200 M Marianas™ Microscope. Cells stained with LIVE/DEAD® Viability/Cytotoxicity Kit. ( A – B ) 2.5× magnification of cultured tissues at day 0, 7 and 14, respectively. ( D – F ) 10× magnification of cultured tissues at day 0, 7 and 14, respectively. Green fluorescence shows live cells, while red fluorescence indicates dead nuclei. Live cells are mainly located central to tissue. ( G) Quantification of live human cells in harvested vascular tissues of distinct patients (N = 9). Box plots show proportions of square micron of green fluorescence divided by the sum of green and red fluorescence. *P < 0.05 compared with other time points using ANOVA with Bonferroni test.

Article Snippet: Viability examination of sections was performed using Zeiss Axiovert 200 M MarianasTM digital imaging inverted microscope system.

Techniques: Immunofluorescence, Microscopy, Staining, Cell Culture, Fluorescence

Journal: Scientific Reports

Article Title: An in vitro method to keep human aortic tissue sections functionally and structurally intact

doi: 10.1038/s41598-018-26549-4

Figure Lengend Snippet: Immunofluorescence images at 10× magnification using Zeiss Axiovert 200 M Marianas™ Microscope. Human tissue stained with LIVE/DEAD® Viability/Cytotoxicity Kit. Green fluorescence shows live cells, while red fluorescence indicates dead nuclei. ( A) Alive tissue at day 62 after harvesting. Tissue viability of 58%. ( B) Alive tissue at day 62 after harvesting at 2.5× magnification and ( C ) at 40× magnification. Outgrowth of new cells is observed after 92 days, while original tissue shows only staining of EthD-1 (dead cells). Tissue viability of 85%. EthD-1 indicates ethdium homodimer-1.

Article Snippet: Viability examination of sections was performed using Zeiss Axiovert 200 M MarianasTM digital imaging inverted microscope system.

Techniques: Immunofluorescence, Microscopy, Staining, Fluorescence

Journal: Scientific Reports

Article Title: An in vitro method to keep human aortic tissue sections functionally and structurally intact

doi: 10.1038/s41598-018-26549-4

Figure Lengend Snippet: Immunofluorescence images showing smooth muscle cells. ( A – C ) Immunofluorescence images at 40× magnification using using Zeiss Axiovert 200 M Marianas™ Microscope at day 5 after harvesting. ( D – F) Immunofluorescence image at 63× magnification using super resolution Confocal Laser Scanning Microscope Leica TCS SP8. Human tissue stained with α-SMA and smoothelin (smooth muscle cell markers) at day 5 after harvesting. ( A , D) Merged image of α-SMA (purple), smoothelin (green) and DAPI (blue). ( B , E) Isolated α-SMA staining. ( C , F) Isolated smoothelin staining. α-SMA indicates alpha smooth muscle actin and DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Viability examination of sections was performed using Zeiss Axiovert 200 M MarianasTM digital imaging inverted microscope system.

Techniques: Immunofluorescence, Microscopy, Laser-Scanning Microscopy, Staining, Isolation

Journal: Scientific Reports

Article Title: An in vitro method to keep human aortic tissue sections functionally and structurally intact

doi: 10.1038/s41598-018-26549-4

Figure Lengend Snippet: Immunofluorescence image at 10× ( A , C ) and 40× ( B , D ) magnification using Zeiss Axiovert 200 M Marianas™ Microscope. Human tissue stained with DAPI (blue), CD45 (green, A , B ) and CD68 (white, C , D ) Green staining at the right side ( A , B ) is due to unintentional fluorescence of elastin. CD indicates cluster of differentiation and DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Viability examination of sections was performed using Zeiss Axiovert 200 M MarianasTM digital imaging inverted microscope system.

Techniques: Immunofluorescence, Microscopy, Staining, Fluorescence

Journal: Scientific Reports

Article Title: An in vitro method to keep human aortic tissue sections functionally and structurally intact

doi: 10.1038/s41598-018-26549-4

Figure Lengend Snippet: Immunofluorescence images at 10× ( A , B ) and 63× oil ( C ) magnification using Zeiss Axiovert 200 M Marianas™ Microscope. ( A ) After 14 days of culturing, tissues were enzymatically digested using collagenase. Live separate cells, stained with Calcein AM (green), float as round cells in culture medium. ( B) After 24 hours of additional culturing, the cells were attached to the slide. ( C) Different cell type characteristics and differences in cell nuclei are observed in attached cells. Live cytoplasm is stained with Calcein AM (green) and cell nuclei are stained with DAPI (blue). AM indicates acetoxymethyl and DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Viability examination of sections was performed using Zeiss Axiovert 200 M MarianasTM digital imaging inverted microscope system.

Techniques: Immunofluorescence, Microscopy, Staining